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Ct (the CMV homolog of HSV gL) results in association of the two proteins and secretion from a transfected cell. This issue may need to be revisited for CMV, since it was recently reported that a third protein, gp 145, coassociates with gH-gL in CMV-infected cells (28). Purification of the HSV-1 gHt-gL complex produced by HL-7 cells was accomplished by immunosorbent chromatography, and the complex
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Nterestingly, all of the gL found in the supernatant was complexed to gHt, arguing for some type of regulation in the secretion process of these two proteins. gHt-gL stimulates a protective immune response. Previous attempts to immunize animals with gH alone (15, 20, 21, 46) or gL alone (3, 21) failed to induce virus neutralizing activity. These failures are now understood on the basis that an int
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Nterestingly, all of the gL found in the supernatant was complexed to gHt, arguing for some type of regulation in the secretion process of these two proteins. gHt-gL stimulates a protective immune response. Previous attempts to immunize animals with gH alone (15, 20, 21, 46) or gL alone (3, 21) failed to induce virus neutralizing activity. These failures are now understood on the basis that an int
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Nterestingly, all of the gL found in the supernatant was complexed to gHt, arguing for some type of regulation in the secretion process of these two proteins. gHt-gL stimulates a protective immune response. Previous attempts to immunize animals with gH alone (15, 20, 21, 46) or gL alone (3, 21) failed to induce virus neutralizing activity. These failures are now understood on the basis that an int
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W that a region of gH just prior to the transmembrane region is critical for function. Several criteria suggest that the truncated soluble complex that we are studying reflects the actual structure of the complex in the virion envelope. First, the gHt-gL complex was antigenically intact, as judged by its capacity to bind to MAbs 52S and 53S, which recognize gH conformation (49), as well as to the
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S for mice immunized with either gD or gHt-gL were lower than those of sham-immunized mice. Of most significance was the finding that all of the sham-immunized mice that developed primary lesions went on to develop severe secondary zosteriform lesions. In contrast, mice immunized with either gD or with gHt-gL exhibited no secondary lesions, regardless of whether they developed any evidence of prim
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Recombinant vaccinia virus (3). Those results are particularly puzzling since it was shown that the expressed gH-gL complex contained the LP11 epitope, considered to be an excellent prognosticator of proper gH-gL conformation (29). One possibility is that the level of gH-gL expression was too low to induce a sufficient immune response. Another possibility is that the assay used in this study has h
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Recombinant vaccinia virus (3). Those results are particularly puzzling since it was shown that the expressed gH-gL complex contained the LP11 epitope, considered to be an excellent prognosticator of proper gH-gL conformation (29). One possibility is that the level of gH-gL expression was too low to induce a sufficient immune response. Another possibility is that the assay used in this study has h