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The cytoplasmic or periplasmic side of the membrane, we attempted to determine the topological organization of TatE using protease accessibility measurements. SpheroplastsMARCH 2, 2012 ?VOLUME 287 ?NUMBERFIGURE 9. Topological organization of TatE probed by protease accessibility assay. Spheroplasts were prepared from E. coli tatAE cells expressing TatE and TatA (from pBAD-E-Strep and pBAD-A-Strep

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